THE PURIFICATION OF TOXIN FROM CLOSTRIDIUM BOTULINUM TYPE A

Purification and activation of Clostridium botulinum type E toxin.

Type E strains of Clostridium botulinum tend to produce toxin of relatively low mouse killing power (administered intraperitoneally) in relation to its high lethality per os for man. The explanation of this peculiarity seems to lie in the phenomenon of "activation," whereby type E toxin, when exposed at an appropriate pH in vitro to certain bacterial enzymes (Dolman, 1953, 1957a; Sakaguchi and ...

Purification and properties of Clostridium botulinum type F toxin.

Clostridium botulinum type F toxin of proteolytic Langeland strain was purified. Toxin in whole cultures was precipitated with (NH4)2SO4. Extract of the precipitate was successively chromatographed on diethylaminoethyl-cellulose at pH 6,0, O-(carboxymethyl) cellulose at pH 4.9, and finally diethylaminoethyl-cellulose at pH 8.1. The procedure recovered 14 percent of the toxin assayed in the star...

Purification and crystallization of Clostridium botulinum type C toxin.

The Purification of Toxin from Ct,ostr.idiijm Botulinum Type A

Until recently it could be said that the status of our knowledge of bacterial exotoxins had not changed since Roux and Yersin in 1888 (1) first obt,ained the filtrable diphtheria toxin. Indeed, Topley and Wilson in 1936 (2) stated, “We have not yet. succeeded in isolating any bacterial esotoxin in a chemically pure state, although a considerable degree of ooncentration has been attained by vari...

Purification of Clostridium botulinum type A neurotoxin.